The Rabbit Anti-Myosin Light Chain 2 Antibody from MyBioSource.com is a Rabbit Polyclonal antibody to MYL2, and myosin light chain 2. This antibody recognizes Human, Mouse, Rat, and Drosophila/Arthropod antigen. The Rabbit Anti-Myosin Light Chain 2 Antibody has been validated for the following applications: Immunocytochemistry, Immunofluorescence, and Western Blot.
Description
Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC or RLC, LC20), has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner (1). The phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fiber (3). Phosphorylation of smooth muscle MLC2 at Ser1/Ser2, and Ser9 by PKC and cdc2, is also reported, which inhibits myosin ATPase activity (4,5). Phosphorylation by cdc2 controls the timing of cytokinesis (5). Lack of phosphorylation sites on cardiac muscle isoform shows morphological and functional abnormalities on transgenic mice (6)